Journal: Journal of Cellular and Molecular Medicine

Article Title: Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice

doi: 10.1111/j.1582-4934.2011.01261.x

Figure Lengend Snippet: Gene symbol, assay ID and RefSeq for RT-PCR

Article Snippet: Mouse Nox2, Nox4 and ANP proteins were examined using purified anti-gp91phox (BD Biosciences, San Diego, CA, USA), anti-Nox4 antibody (Novus Biologicals, Littleton, CO, USA) and anti-ANP (Santa Cruz Biotechnology Inc.), following the procedures previously described [ ].

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice

doi: 10.1111/j.1582-4934.2011.01261.x

Figure Lengend Snippet: Upregulation of Nox2 and Nox4 mRNA and protein in ventricles of TNF-α-injected mice. Total RNA was extracted from the ventricular tissues of TNF-α-injected and control mice and Nox2 and Nox4 mRNA levels were examined using real-time PCR. Nox2 (A) and Nox4 mRNA levels (B) were shown. Nox2 and Nox4 protein levels were determined by immunoblotting. A representative of Nox2 and Nox4 proteins were shown (C). Densitometric analysis of Nox2 (D) and Nox4 protein (E) normalized with GAPDH were shown. C, control; T, TNF-α-injected mice. * P < 0.05.

Article Snippet: Mouse Nox2, Nox4 and ANP proteins were examined using purified anti-gp91phox (BD Biosciences, San Diego, CA, USA), anti-Nox4 antibody (Novus Biologicals, Littleton, CO, USA) and anti-ANP (Santa Cruz Biotechnology Inc.), following the procedures previously described [ ].

Techniques: Injection, Control, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice

doi: 10.1111/j.1582-4934.2011.01261.x

Figure Lengend Snippet: Nox2 and Nox4 mediate TNF-α-induced upregulation of IL-1β and IL-6 genes and ROS production. Human adult cardiomyocytes were transfected with Nox2 and Nox4 siRNA using siPORT Amine transfection reagents. Total RNA was extracted from human adult cardiomyocytes 24 hrs after transfection. Human Nox2 and Nox4 mRNA levels were examined using real-time PCR. Nox2 (A) and Nox4 (B) mRNA levels after siRNA treatment were shown. In the other experiments, cells were treated with TNF-α (20 ng/ml) for 6 hrs at 37°C after treatment with Nox2 and Nox4 siRNA. IL-1β (C) and IL-6 mRNA (D) levels were examined with real-time PCR. TNF-α-induced ROS production in human adult cardiomyocytes was determined with CM-H 2 DCFDA following Nox2 and Nox4 siRNA treatment (E). Data were expressed as relative light unit (RLU) ± S.D. * P < 0.05 versus control values. # P < 0.05 versus TNF-α treatment values.

Article Snippet: Mouse Nox2, Nox4 and ANP proteins were examined using purified anti-gp91phox (BD Biosciences, San Diego, CA, USA), anti-Nox4 antibody (Novus Biologicals, Littleton, CO, USA) and anti-ANP (Santa Cruz Biotechnology Inc.), following the procedures previously described [ ].

Techniques: Transfection, Real-time Polymerase Chain Reaction, Control